BPC 157 (Body Protection
Compound-157) is a pentadecapeptide made up of 15 amino acids. The amino acids
sequence in BPC 157 is similar to a portion of the human BPC amino acid
sequence. Human BPC is found in the gastric juice. Experiments have shown that
BPC 157 enhances the healing of wounds, including tendons wounds such as
transected Achilles tendons of rats. The aim of this study was to investigate
the probable mechanism that BPC 157 utilizes to accelerate the healing process
in an injured tendon. The study used two group of tendon explants of which one
group was cultured in a BPC 157-containing medium while the other group was
cultured in a medium lacking BPC 157. These cultures were thereafter examined
for tendon fibroblasts outgrowths. Such outgrowths indicated tendon
regeneration.The results revealed that the explants’ outgrowth was
significantly accelerated in the culture containing BPC 157 as compared to the
culture lacking BPC 157. Also, a MTT assay did show that BPC 157 does not
directly affect cellular proliferation in a culture of rat-derived Achilles tendon.
However, results also showed that BPC 157 significantly increased the survival
of cells under oxidative stress. Furthermore, the Transwell filter migration
assay showed that BPC 157 significantly increased in-vitro fibroblast migration
in a dose-dependent fashion. Moreover, BPC 157 accelerated the dispersal of the
fibroblasts in culture dishes in a dose-dependent manner.
Additionally,
FITC-phalloidin staining was able to demonstrate that BPC 157 induces F-actin
formation in fibroblasts. Likewise, Western blot analysis was able to detect
the production and activation of paxillin and FAK proteins. The western blot
analysis also showed that BPC 157 increases the extent of phosphorylation of
paxillin and FAK proteins without affecting the amounts produced.
Thus, it can be concluded that BPC 157 enhances the ex-vivo growth and in-vitro cellular migration of fibroblasts derived from rat tendon explants. Moreover, BPC 157 also increases the probability of a cell surviving under oxidative stress. These actions of BPC 157 are probably mediated by the activation (through phosphorylation) of the proteinic FAK-paxillin pathway.
Molecular formula: C62H98N16O22
Molar Mass:1419.53552 g/mol
CAS: 137525-51-0
BPC 157 is available for research needs. It is not accessible for human use and continues to be a popular research chemical. It should ideally be handled by professionals who are aware f the safety concerns and benefits that the product provides for research purposes.
Lyophilized peptides
although stable at room temperature for 3 months, should be stored dessicated
below -18 degrees Celsius. Upon reconstitution of the peptide it should be
stored at 4 degrees Celsius between 2-21 days and for future use below -18 degrees
Celsius.
Peptex Laboratories Products
are sold strictly for research purposes only. Please do not ask about human
consumption as this is strictly forbidden.